The HIV fourth-generation test looks for p24 antigen along with antibodies to HIV 1 and HIV 2. This is considered highly accurate after 28 days and is taken as conclusive after 45 days of suspected exposure.

In your case, the test is negative after 25 days; therefore, you are reasonably safe and must not worry.

As per protocol , it is advisable to repeat the test after 45 days.

For more ask a doctor