Yes, you can read the Hanuman Chalisa during menstruation. In modern understanding, menstruation is a natural biological process, and there are no inherent spiritual restrictions related to it. Many spiritual teachers and scholars emphasize that devotion, faith, and intent are what truly matter in worship, rather than external conditions.

While some traditional practices in certain cultures may have placed restrictions on menstruation regarding rituals, many people today believe these customs should not prevent someone from engaging in prayer or devotion. Ultimately, it's a personal choice based on one's beliefs and comfort level.